Download E-books Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols PDF

By Salvatore A. E. Marras (auth.), Vladimir V. Didenko MD, PhD (eds.)

Although fluorescent probes and assays, which use power move (ET) for tracking DNA reactions, have elevated lately, formerly there were no manuals summarizing the various various protocols and probe designs. Fluorescent strength move Nucleic Acid Probes: Designs and Protocols is the 1st book to supply this sort of assortment. within the quantity, hands-on experts-often the unique builders of the technique-comprehensively describe numerous fluorescent probes and units that use ET, together with molecular beacons, molecular holiday lighting fixtures, TaqMan® probes, LUX™ primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and good judgment gates for molecular-scale computation. Merging paintings on nanotechnology and fluorescent probes, the authors gather the 1st complete therapy of the entire key DNA- and RNA-based ET probes and current ideas for his or her optimized customized layout. They talk about either fluorescence resonance power move (FRET)-based and non-FRET-based constructs, and supply an entire set of suggestions to observe DNA and RNA reactions, reminiscent of hybridization, amplification, cleavage, folding, and institutions with proteins, different molecules, and steel ions. certain protocols are supplied for distance selection in protein-DNA complexes and the detection of topological DNA adjustments, mutations, and single-nucleotide polymorphisms. The protocols keep on with the profitable tools in Molecular Biology™ sequence structure, each one delivering an summary of the foundations in the back of the method, step by step distinctive directions, and complete troubleshooting.
Exhaustive and cutting-edge, Fluorescent strength move Nucleic Acid Probes: Designs and Protocols is a useful source for either amateur and skilled researchers who desire to use the most recent advancements in fluorescent probes in any box of molecular biology.

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